Speaker：Dr. Jianxiong Ye

                       Fujian Normal University

Title:  An ensemble dynamic flux modelling of glycerol metabolism in Klebsiella pneumoniae

       Time：26 Oct 2019, 10:00

       Location：物机大楼661

Abstract: Computational models for the determination of in vivo metabolic fluxes provide great insight into the understanding of cellular metabolism, and provide guidance for design, control and optimization of biotechnological processes. In this paper, an ensemble modelling framework is proposed for the determination of the dynamic flux distribution of glycerol metabolic pathways in Klebsiella pneumoniae. This integrated model consists of two different kinetic models for extracellular concentrations and a constraint-based model for intracellular fluxes. The extracellular kinetic models provide time-varying input fluxes for the intracellular system, resulting in a dynamic evaluation of intracellular flux distribution from non-steady state to steady state in continuous culture. The objective function in the constraint-based intracellular model is inferred through a bilevel programming problem. The computed fluxes are in consistent with experimental data under both substrate-limited and substrate-excess cultures. The simulated results also show the profiles of the intermediate fluxes. They help to understand the particular metabolism, serving as a theoretical guide for the optimization of theses processes.

Speaker  Introduction：叶剑雄，福建师范大学数学与信息学院副教授，福建省运筹学协会理事，研究方向为最优控制理论及应用，目前从事生物系统的优化和控制，2019年应邀加入国际基因工程机器大赛（iGEM）测量委员会。2011年大连理工大学博士毕业，2011年至今工作于福建师范大学。2013年-2015年期间在华中科技大学从事博士后研究，2018年-2019年在澳大利亚Curtin大学进行学术访问。迄今发表学术论文30余篇，其中SCI检索20余篇；主持国家自然科学基金1项，省自然科学基金3项，省高校杰出青年计划1项。

        联系人：李安教授